One-step microfluidics production of enzyme-loaded liposomes for the treatment of inflammatory diseases

The biopharmaceuticals market is constantly growing. Despite their advantages over the conventional drugs, have short biological half-lifes, which can be increased using liposomes. However, common bulk methods to produce biopharmaceuticals-loaded liposomes result in lost of encapsulation efficiency (E.E.), resulting an expensive process. Herein, a therapeutic enzyme proposed, glass-capillary microfluidic technique. Cu,Zn- Superoxide dismutase (SOD) successfully encapsulated into ([email protected]). [email protected] with mean size 135 ± 41 nm, polydispersity index 0.13 0.01, E.E. 59 6 % and activity 82 3 are obtained. vivo experiments show, through ear edema model, that administered by intravenous route enable inhibition 65 8 %, 20 13 SOD its free form. histopathological analyses show higher inflammatory cell accumulation on treated form, than Overall, this work highlights potential microfluidics for production enzyme-loaded high efficiency, intrinsic low time-consuming easily upscaling assembly method.